Property persistence of «kerosene» fungus hormoconis resinae strains during long-term storage in the laboratory

Krivushina A.A., Bobyreva T.V.
Krivushina A.A., Bobyreva T.V. Property persistence of «kerosene» fungus hormoconis resinae strains during long-term storage in the laboratory // Proceedings of VIAM. 2019. No. 11. DOI: 10.18577/2307-6046-2019-0-11-104-112. URL: https://test.viam.ru/en/journal/2019/11/12
Keywords
Hormoconis resinae, Cladosporium resinae, biodeterioration, aviation fuel, microbiological resistance, fungi resistance of fuel, microbial destruction.
Abstract

The property persistence of Hormoconis resinae (or «kerosene» fungus), seven strains was studied after many years of storage in the collection by subculture on nutrient media. It was shown that all strains retained their ability to grow due to hydrocarbon aviation fuel, but the lag phase increased significantly (or the time required to starting active growth in fuel) – from 1 to 3 months. Lag phase of re-isolated strains deceased to 10–14 days. These strains were deposited in the FSUE «VIAM» museum collection of microorganisms by lyophilization and cryogenic freezing. Since the fungal resistance tests duration according to GOST 9.023–74 is 21 days, the results should be taken into account when choosing a storage method and cultivation conditions before testing.

Reference list
  1. Koval E.Z., Sidorenko L.P. Mikodestruktory promyshlennykh materialov [Microdestructors of industrial materials]. Kiev: Naukova dumka, 1989. 187 s.
  2. Hamme J.D.V., Singh A., Ward O.P. Recent advances in petroleum microbiology // Microbiology and molecular biology reviews. 2003. No. 67 (4). P. 503–549.
  3. Rauch M.E., Graef H.W., Rozenzhak S.M. et al. Characterization of Microbial Contamination in United States Air Force Aviation Fuel Tanks // Journal of Industrial Microbiology and Biotechnology. 2006. No. 33 (1). P. 29–36.
  4. Semenov S.A., Gumargalieva K.Z., Zaikov G.E. Biopovrezhdeniya materialov i izdeliy tekhniki // Gorenie, destruktsiya i stabilizatsiya polimerov [Biodeterioration of materials and products of technology]. SPb.: Nauchnyye osnovy i tekhnologii, 2008. C. 73–99.
  5. Kablov E.N. Klyuchevaya problema – materialy [The key problem is materials] // Tendentsii i oriyentiry innovatsionnogo razvitiya Rossii. M.: VIAM, 2015. S. 458–464.
  6. Kablov E.N., Erofeev V.T., Svetlov D.A., Smirnov V.F., Bogatov A.D. Biopovrezhdeniya v kosmicheskikh apparatakh [Biodeterioration in spacecraft] // Sb. dokl. Mezhdunar. nauch.-tekhn. konf. «Kompozitsionnyye materialy. Teoriya i praktika». Penza, 2015. S. 40–46.
  7. Crous P.W., Braun U., Schubert K., Groenewald J.Z. Delimiting Cladosporium from morphologically similar genera. Studies in Mycology. 2007. No. 58. P. 33–56.
  8. Kondratyuk T.A., Kharkevich E.S., Zakharchenkova V.A. i dr. Biopovrezhdeniye aviatsionnogo topliva TS-1 mikroskopicheskimi gribami [Biological damage to aviation fuel TS-1 by microscopic fungi] // Mikologiya i fitopatologiya. 2007. T. 41. №5. S. 442–448.
  9. McVea G.G., Solly R.K. Control of fuel microorganisms with magnetic devices: laboratory investigation with Hormoconis resinae // Aircraft Materials Technical Memorandum. 1991. No. 408. P. 1–11.
  10. Martin-Sanchez P.M., Gorbushina A.A., Kunte H.J., Toepel J. A novel QPCR protocol for the specific detection and quantification of the fuel-deteriorating fungus Hormoconis resinae. Biofouling. Taylor&Francis. 2016. Vol. 32. No. 6. P. 635–644.
  11. GOST 9.023–74. ESZKS. Topliva neftyanyye. Metod laboratornykh ispytaniy biostoykosti topliv, zashchishchennykh protivomikrobnymi prisadkami. Zashchita ot korrozii. Chast 6. Zashchita ot biopovrezhdeniy [Unified system of corrosion and ageing protection. Oil fuels. Method of laboratory testing biostability of fuels protected by antimicrobe additives. Corrosion protection. Part 6. Protection against biodeterioration]. M.: Izd-vo standartov. 1994. 9 s.
  12. Kablov E.N., Startsev V.O. Sistemnyj analiz vliyaniya klimata na mekhanicheskie svojstva polimernykh kompozitsionnykh materialov po dannym otechestvennykh i zarubezhnykh istochnikov (obzor) [Systematical analysis of the climatics influence on mechanical properties of the polymer composite materials based on domestic and foreign sources (review)] // Aviacionnye materialy i tehnologii. 2018. №2 (51). S. 47–58. DOI: 10.18577/2071-9140-2018-0-2-47-58.
  13. Krivushina A.A., Goryashnik Yu.S. Sposoby zashchity materialov i izdeliy ot mikrobiologicheskogo porazheniya (obzor) [Ways of protection of materials and products from microbiological damage (review)] // Aviacionnye materialy i tehnologii. 2017. №2 (47). S. 80–86. DOI: 10.18577/2071-9140-2017-0-2-80-86.
  14. Kablov E.N. Innovacionnye razrabotki FGUP «VIAM» GNC RF po realizacii «Strategicheskih napravlenij razvitiya materialov i tehnologij ih pererabotki na period do 2030 goda» [Innovative developments of FSUE «VIAM» SSC of RF on realization of «Strategic directions of the development of materials and technologies of their processing for the period until 2030»] // Aviacionnye materialy i tehnologii. 2015. №1 (34). S. 3–33. DOI: 10.18577/2071-9140-2015-0-1-3-33.
  15. Polyakova A.V., Krivushina A.A., Goryashnik Yu.S., Buharev G.M. Ispytaniya na mikrobiologicheskuyu stojkost v naturnyh usloviyah razlichnyh klimaticheskih zon [Microbiological resistance tests under nature conditions in variety of climatiс zones] // Trudy VIAM: elektron. nauch.-tehnich. zhurn. 2016. №4. St. 11. Available at: http://www.viam-works.ru (accessed: March 25, 2019). DOI: 10.18577/2307-6046-2016-0-4-11-11.
  16. Vainio A.E.I. Amylolytic yeast: expression of Hormoconis resinae glucoamylase P in Saccharomyces cerevisiae. PhD Thesis. Turun Yliopisto Institute, Finland, 1996. Р. 3–8.
  17. Morgan P., Watkinson R.J. Biodegradation of components of petroleum. Biochemistry of microbial degradation. 1994. P. 1–31.
  18. Rafin C., Veignie E. Hormoconis resinae, the kerosene fungus: Handbook of hydrocarbon and lipid microbiology. Springer International Publishing AG, 2018. Р. 16–21.
  19. Krivushina A.A., Chekunova L.N., Mokeyeva V.L. Morfologicheskiye osobennosti shtammov «kerosinovogo» griba Hormoconis resinae pri roste v aviatsionnom toplive i na pitatelnykh sredakh [Morphological features of strains of the «kerosene» fungus Hormoconis resinae when grown in aviation fuel and on nutrient media] // Mikologiya i fitopatologiya. 2019. №1. S. 23–32.
  20. Domsch K.H., Gams W., Anderson T.H. Compendium of Soil Fungi (2nd ed.). Lubrecht & Cramer Ltd, 2007. 672 p.
  21. Tsavkelova E.L., Klimova S.Yu., Cherdyntseva T.L., Netrusov L.I. Mikroorganizmy – produtsenty stimulyatorov rosta rasteniy i ikh prakticheskoye primeneniye (obzor) [Microorganisms - producers of plant growth stimulants and their practical application (review) ] // Prikladnaya biokhimiya i mikrobiologiya. 2006. T. 42. №2. S. 133–143.