Identification of the mycelial fungi collection of NRC «Kurchatov Institute» – VIAM by molecular genetic methods

Part 1
Krivushina A.A., Startsev V.O.
Krivushina A.A., Startsev V.O. Identification of the mycelial fungi collection of NRC «Kurchatov Institute» – VIAM by molecular genetic methods. Part 1 // Proceedings of VIAM. 2026. No. 4. DOI: 10.18577/2307-6046-2026-0-4-183-192. URL: https://test.viam.ru/en/journal/2026/4/15
Keywords
biodeterioration, taxonomic identification, collection of microorganisms, destructive microorganisms, microbiological resistance, microbiological damage, micromycetes, molecular diagnostics, sequencing, phylogenetic analysis
Abstract

The taxonomic identification of mycelial fungal strains from the collection of the NRC «Kurchatov Institute» – VIAM was carried out using methods of genome-wide taxonomy. The taxonomic classification was performed at the Kurchatov Genomic Center using two approaches: genomic signature analysis and a full-fledged phylogenetic analysis of 255 marker single-copy eukaryotic genes. This article presents data from phylogenetic analysis of strains based on sequences of universal eukaryotic marker genes of the following fungi: order Glomerellales, genus Colletotrichum; the family Pleosporaceae; the genera Curvularia and Alternaria, the genus Aspergillus, and representatives of new orders of the class Sordariomycetes.

Reference list
  1. Kablov E.N., Laptev A.B., Prokopenko A.N., Gulyaev A.I. Relaxation of polymeric composite materials under the prolonged action of static load and climate (review). Part 1. Binders. Aviation materials and technologies, 2021, no. 4 (65), pp. 70–80. Available at: http://www.journal.viam.ru (accessed: July 20, 2025). DOI: 10.18577/2713-0193-2021-0-4-70-80.
  2. Zakirova L.I., Afanasyev-Khodykin A.N., Movenko D.A., Laptev A.B. Features of the formation of the Sn–Zn–Fe diffusion layer at the boundary of galvanothermal coating of systems zinc–tin and 30HGSA steel with high protec-tive capability. Aviation materials and technologies, 2022, no. 4 (69), pp. 61–71. Available at: http://www.journal.viam.ru (accessed: July 29, 2025). DOI: 10.18577/2713-0193-2022-0-4-61-71.
  3. Startsev V.O., Startsev O.V., Zeleneva T.O., Vardanyan A.M. Influence of precipitation on changes in the mass of samples of polymeric composite materials in open climatic conditions. Aviation materials and technologies, 2024, no. 1 (74), pp. 136–154. Available at: http://www.journal.viam.ru (accessed: July 20, 2025). DOI: 10.18577/2713-0193-2024-0-1-136-154.
  4. Lugauskas A., Levinskait L., Pečiulyt D. Micromycetes as deterioration agents of polymeric materials. International biodeterioration & biodegradation, 2003, vol. 52 (4), pp. 233–242.
  5. Lugauskas A., Prosychevas I., Levinskaitė L., Jaskelevičius B. Physical and chemical aspects of long‐term biodeterioration of some polymers and composites. Environmental Toxicology: An International Journal, 2004, vol. 19 (4), pp. 318–328.
  6. Srikanth M., Sandeep T.S.R.S., Sucharitha K., Godi S. Biodegradation of plastic polymers by fungi: a brief review. Bioresources and Bioprocessing, 2022, vol. 9 (1), p. 42. DOI: 10.1186/s40643-022-00532-4.
  7. Krivushina A.A., Laptev A.B. Fungicides: application, properties and principles of action. Aviation materials and technologies, 2024, no. 1 (74), pp. 155–168. Available at: http://www.journal.viam.ru (accessed: July 20, 2025). DOI: 10.18577/2713-0193-2024-0-1-155-168.
  8. Kablov E.N., Antipov V.V. The role of new generation materials in ensuring the technological sovereignty of the Russian Federation. Vestnik Rossiyskoy akademii nauk, 2023, vol. 93, no. 10, pp. 907–916. DOI: 10.31857/S0869587323100055.
  9. Jeon S.A., Park J.L., Park S.J. et al. Comparison between MGI and Illumina sequencing platforms for whole genome sequencing. Genes and Genomics, 2021, vol. 43, no. 7, pp. 713–724. DOI: 10.1007/s13258-021-01096-x.
  10. Hu T., Che J., Lin X. et al. Comparison of the DNBSEQ platform and Illumina HiSeq 2000 for bacterial genome assembly. Scientific Reports, 2024, vol. 14, no. 1, p. 1292. DOI: 10.1038/s41598-024-51725-0.
  11. Meslier V., Quinquis B., Da Silva K. et al. Benchmarking second and third-generation sequencing platforms for microbial metagenomics: 1. Scientific Data, 2022, vol. 9, no. 1, p. 694.
  12. Blanco-Míguez A., Beghini F., Cumbo F. et al. Extending and improving metagenomic taxonomic profiling with uncharacterized species using MetaPhlAn 4. Nature biotechnology, 2023, vol. 41 (11), pp. 1633–1644.
  13. Manni M., Berkele M.R., Seppey M. et al. BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Molecular biology and evolution, 2021, vol. 38, no. 10, pp. 4647–4654.
  14. Katoh K., Standley D.M. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular biology and evolution, 2013, vol. 30, no. 4, pp. 772–780.
  15. Capella-Gutiérrez S., Silla-Martínez J.M., Gabaldón T. TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics, 2009, vol. 25, no. 15, pp. 1972–1973.
  16. Price M.N., Dehal P.S., Arkin A.P. FastTree 2 – approximately maximum-likelihood trees for large alignments. PLOS One, 2010, vol. 5, no. 3, p. e9490.