Isolation of bacterial strains of polymer materials destructors from samples of recycled cooling water from oil refineries and petrochemical plants

Ermishev V.Yu.
UDC
579.017.8
DOI
10.18577/2307-6046-2025-0-4-107-128
Article PDF (Russian) (2.46 MB)
How to cite
Ermishev V.Yu. Isolation of bacterial strains of polymer materials destructors from samples of recycled cooling water from oil refineries and petrochemical plants // Proceedings of VIAM. 2025. No. 4. DOI: 10.18577/2307-6046-2025-0-4-107-128. URL: https://test.viam.ru/en/journal/2025/4/9
Keywords
Pseudomonas, biodegradation, bacterial strains, synthetic polymer and composite materials, fuel, sequencing, 16S rRNA
Abstract

The article presents methods for isolating bacterial strains of potential destructors of polymer materials, using the example of recycled water samples from enterprises producing petroleum products. The cultures were exposed with polymer materials and polymer substrates. Data on the species composition of bacterial cultures were obtained as a result of DNA sequencing of a fragment encoding the V4 site of the 16S rRNA of bacteria. The obtained pure cultures of bacteria of the genus Pseudomonas were used to obtain comparative growth kinetics in a mineral medium with the addition of jet and diesel fuel.

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